Specific Binding of Integrase to the Origin of Transfer ( oriT ) of the Conjugative Transposon Tn 916

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چکیده

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Specific binding of integrase to the origin of transfer (oriT) of the conjugative transposon Tn916.

Purified integrase protein (Int) of the conjugative transposon Tn916 was shown, using nuclease protection experiments, to bind specifically to a site within the origin of conjugal transfer of the transposon, oriT. A sequence similar to the ends of the transposon that are bound by the C-terminal DNA-binding domain of Int was present in the protected region. However, Int binding to oriT required ...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 2001

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.183.9.2947-2951.2001